Abstract Detail



Poster Session

Conner, James N [1], Babos, Marybeth [2], Henderson, Melissa [3].

Examining Non-Lethal Doses of Alpha Amanitin in C. elegans.

Amatoxins are a subgroup of at least eight toxic cyclic octapeptides that occur in several genera of mushroom, including those of the genus Amanita.   Human ingestion of these mushrooms is usually fatal, with toxicity manifesting in tissues that possess high rates of protein synthesis due to the ability of the toxins to inhibit RNA polymerase II.   We studied the cellular effects of the constituent alpha amanitin using the model organism Caenorhabditis elegans.  C.elegans, a nematode known for demonstrating genetic and molecular effects analogous to humans, is inherently sensitive to the amatoxins including alpha-amanitin.  We examined the effect of the toxin in non-lethal concentrations to determine the influence on larval growth and development, on the induction of apoptosis in the germline of C.elegans,  and on the level of gene expression of several mRNAs involved in apoptosis.   Further, we compared the effects of commercially available amanitin to a crude extract derived from Amanita virosa in order to determine whether there is an “entourage effect” from other constituents in the fungus. Our studies have outlined a novel approach to examine the actions of nature-derived compounds using C.elegans as a model. 


1 - Lincoln Memorial University-DeBusk College of Osteopathic Medicine, 6965 Cumberland Gap Parkway, Harrogate, TN, 37752, USA
2 - Lincoln Memorial University-DeBusk College of Osteopathic Medicine, Pharmacology and Physiology, 6965 Cumberland Gap Parkway, Harrogate, TN, 37752, USA
3 - Lincoln Memorial University-DeBusk College of Osteopathic Medicine, Molecular Sciences, 6965 Cumberland Gap Parkway, Harrogate, TN, 37752, USA

Keywords:
Amanitin.

Presentation Type: Poster
Number:
Abstract ID:52
Candidate for Awards:Julia F. Morton Award