Abstract Detail



Poster Session

Dexter, Kenneth [1], Babos, Marybeth [2], Henderson, Melissa [3].

Luteolin and its effect on V-ATPase activity in C.elegans.

     Luteolin is a naturally occurring flavonoid that is sold as an over the counter dietary supplement.  Plants rich in luteolin have long been used in Chinese medicine to treat a variety of ailments.  Recent research has shown that luteolin inhibits the V-ATPase enzyme in cells.  V-ATPase is a proton pump that works both on the cell surface and on the surface of many organelles including endosomes, lysosomes, and secretory vesicles.  It acts by using energy from ATP to create a transmembrane hydrogen ion gradient which is often used to drive many secondary active transports.  Although luteolin is said to have a number of health benefits, the direct mechanism of action is unknown.  We have used the model organism Caenorhabditis elegans to further investigate how luteolin affects the V-ATPase.  Here we demonstrate the effect of luteolin on the V-ATPase on the growth and development of the nematode, its brood size, and the function of the V-ATPase in the sperm secretory vesicles.  This study contributes to the establishment of C. elegans as a screening model for compounds found in medicinal herbs.  


1 - Lincoln Memorial University - DeBusk College of Osteopathic Medicine, 6965 Cumberland Gap Pkwy, Harrogate, TN, 37752, USA
2 - Lincoln Memorial University - DeBusk College of Osteopathic Medicine, Pharmacology and Physiology, 6965 Cumberland Gap Pkwy, Harrogate, TN, 37752, USA
3 - Lincoln Memorial University - DeBusk College of Osteopathic Medicine, Molecular Sciences, 6965 Cumberland Gap Pkwy, Harrogate, TN, 37752, USA

Keywords:
Luteolin.

Presentation Type: Poster
Number:
Abstract ID:46
Candidate for Awards:Julia F. Morton Award